Construct: shRNA TRCN0000070331

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485785.1-448s1c1
DNA Barcode:
CCTTTGCCTATGGGCTAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC434043 (434043)
Gene Description:
similar to solute carrier family 5 (sodium-dependent vitamin transporter), member 6
Transcript:
RefSeq XM_485785.1 (NON-CURRENT)
Match location:
Position 448 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070331)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGCCTATGGGCTAGTTT
Hairpin Sequence:
5'-CCGG-CCTTTGCCTATGGGCTAGTTT-CTCGAG-AAACTAGCCCATAGGCAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGCCTATGGGCTAGTTTCTCGAGAAACTAGCCCATAGGCAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGCCTATGGGCTAGTTTCTCGAGAAACTAGCCCATAGGCAAAGG-3'

Other clones with same target sequence:

(none)