Construct: shRNA TRCN0000070359
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_028122.2-299s1c1
- DNA Barcode:
- CTCCAGTTCATGGACTGGATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Slc14a1 (108052)
- Gene Description:
- solute carrier family 14 (urea transporter), member 1
- Transcript:
- RefSeq NM_028122.2 (NON-CURRENT)
- Match location:
- Position 299 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000070359)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTCCAGTTCATGGACTGGATA
- Hairpin Sequence:
-
5'-CCGG-CTCCAGTTCATGGACTGGATA-CTCGAG-TATCCAGTCCATGAACTGGAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTCCAGTTCATGGACTGGATACTCGAGTATCCAGTCCATGAACTGGAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTCCAGTTCATGGACTGGATACTCGAGTATCCAGTCCATGAACTGGAG-3'
Other clones with same target sequence:
(none)
