Construct: shRNA TRCN0000070363

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_148931.2-5865s1c1
DNA Barcode:
GCACTTTAGAAAGAGTGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc6a5 (104245)
Gene Description:
solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Transcript:
RefSeq NM_148931.2 (NON-CURRENT)
Match location:
Position 5865 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070363)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTTAGAAAGAGTGGAAT
Hairpin Sequence:
5'-CCGG-GCACTTTAGAAAGAGTGGAAT-CTCGAG-ATTCCACTCTTTCTAAAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTTAGAAAGAGTGGAATCTCGAGATTCCACTCTTTCTAAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTTAGAAAGAGTGGAATCTCGAGATTCCACTCTTTCTAAAGTGC-3'

Other clones with same target sequence:

(none)