Construct: shRNA TRCN0000070387

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177263.3-2314s1c1
DNA Barcode:
CGAGTCCTAGAGAACAGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zhx3 (320799)
Gene Description:
zinc fingers and homeoboxes 3
Transcript:
RefSeq NM_177263.3 (CURRENT)
Match location:
Position 2314 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070387)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGTCCTAGAGAACAGCTTT
Hairpin Sequence:
5'-CCGG-CGAGTCCTAGAGAACAGCTTT-CTCGAG-AAAGCTGTTCTCTAGGACTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGTCCTAGAGAACAGCTTTCTCGAGAAAGCTGTTCTCTAGGACTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGTCCTAGAGAACAGCTTTCTCGAGAAAGCTGTTCTCTAGGACTCG-3'

Other clones with same target sequence:

(none)