Construct: shRNA TRCN0000070395

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199449.1-1297s1c1
DNA Barcode:
GCTGAATACTACCAAATACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zhx2 (387609)
Gene Description:
zinc fingers and homeoboxes 2
Transcript:
RefSeq NM_199449.1 (NON-CURRENT)
Match location:
Position 1297 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070395)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAATACTACCAAATACAA
Hairpin Sequence:
5'-CCGG-GCTGAATACTACCAAATACAA-CTCGAG-TTGTATTTGGTAGTATTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAATACTACCAAATACAACTCGAGTTGTATTTGGTAGTATTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAATACTACCAAATACAACTCGAGTTGTATTTGGTAGTATTCAGC-3'

Other clones with same target sequence:

(none)