Construct: shRNA TRCN0000070405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018885.1-565s1c1
DNA Barcode:
CCAGTACCCATATGACAGGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Irx4 (50916)
Gene Description:
Iroquois related homeobox 4 (Drosophila)
Transcript:
RefSeq NM_018885.1 (NON-CURRENT)
Match location:
Position 565 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTACCCATATGACAGGTA
Hairpin Sequence:
5'-CCGG-CCAGTACCCATATGACAGGTA-CTCGAG-TACCTGTCATATGGGTACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTACCCATATGACAGGTACTCGAGTACCTGTCATATGGGTACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTACCCATATGACAGGTACTCGAGTACCTGTCATATGGGTACTGG-3'

Other clones with same target sequence:

(none)