Construct: shRNA TRCN0000070441

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033652.2-270s1c1
DNA Barcode:
CTCGGCATCTTTCTCCTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lmx1a (110648)
Gene Description:
LIM homeobox transcription factor 1 alpha
Transcript:
RefSeq NM_033652.2 (NON-CURRENT)
Match location:
Position 270 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070441)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGGCATCTTTCTCCTCTTT
Hairpin Sequence:
5'-CCGG-CTCGGCATCTTTCTCCTCTTT-CTCGAG-AAAGAGGAGAAAGATGCCGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGGCATCTTTCTCCTCTTTCTCGAGAAAGAGGAGAAAGATGCCGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGGCATCTTTCTCCTCTTTCTCGAGAAAGAGGAGAAAGATGCCGAG-3'

Other clones with same target sequence:

(none)