Construct: shRNA TRCN0000070524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008498.1-1443s1c1
DNA Barcode:
CGTCCAGTGCTGTGAATGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lhx1 (16869)
Gene Description:
LIM homeobox protein 1
Transcript:
RefSeq NM_008498.1 (NON-CURRENT)
Match location:
Position 1443 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCCAGTGCTGTGAATGTAA
Hairpin Sequence:
5'-CCGG-CGTCCAGTGCTGTGAATGTAA-CTCGAG-TTACATTCACAGCACTGGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCCAGTGCTGTGAATGTAACTCGAGTTACATTCACAGCACTGGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCCAGTGCTGTGAATGTAACTCGAGTTACATTCACAGCACTGGACG-3'

Other clones with same target sequence:

(none)