Construct: shRNA TRCN0000070615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008584.1-809s1c1
DNA Barcode:
GAAGGAAACTACAAGTCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Meox2 (17286)
Gene Description:
mesenchyme homeobox 2
Transcript:
RefSeq NM_008584.1 (NON-CURRENT)
Match location:
Position 809 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGAAACTACAAGTCAGAA
Hairpin Sequence:
5'-CCGG-GAAGGAAACTACAAGTCAGAA-CTCGAG-TTCTGACTTGTAGTTTCCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGAAACTACAAGTCAGAACTCGAGTTCTGACTTGTAGTTTCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGAAACTACAAGTCAGAACTCGAGTTCTGACTTGTAGTTTCCTTC-3'

Other clones with same target sequence:

(none)