Construct: shRNA TRCN0000070652

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010876.2-84s1c1
DNA Barcode:
TGTGTACATGTTCCTGGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ncf1 (17969)
Gene Description:
neutrophil cytosolic factor 1
Transcript:
RefSeq NM_010876.2 (NON-CURRENT)
Match location:
Position 84 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070652)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGTACATGTTCCTGGTTAA
Hairpin Sequence:
5'-CCGG-TGTGTACATGTTCCTGGTTAA-CTCGAG-TTAACCAGGAACATGTACACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGTACATGTTCCTGGTTAACTCGAGTTAACCAGGAACATGTACACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGTACATGTTCCTGGTTAACTCGAGTTAACCAGGAACATGTACACA-3'

Other clones with same target sequence:

(none)