Construct: shRNA TRCN0000070662

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010877.2-1184s1c1
DNA Barcode:
GCCTATGCCTTACATGCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ncf2 (17970)
Gene Description:
neutrophil cytosolic factor 2
Transcript:
RefSeq NM_010877.2 (NON-CURRENT)
Match location:
Position 1184 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070662)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTATGCCTTACATGCTCAA
Hairpin Sequence:
5'-CCGG-GCCTATGCCTTACATGCTCAA-CTCGAG-TTGAGCATGTAAGGCATAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTATGCCTTACATGCTCAACTCGAGTTGAGCATGTAAGGCATAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTATGCCTTACATGCTCAACTCGAGTTGAGCATGTAAGGCATAGGC-3'

Other clones with same target sequence:

(none)