Construct: shRNA TRCN0000070823

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008266.3-337s1c1
DNA Barcode:
CTATGGGCCTTCTCAGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hoxb1 (15407)
Gene Description:
homeobox B1
Transcript:
RefSeq NM_008266.3 (NON-CURRENT)
Match location:
Position 337 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070823)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGGGCCTTCTCAGTATTA
Hairpin Sequence:
5'-CCGG-CTATGGGCCTTCTCAGTATTA-CTCGAG-TAATACTGAGAAGGCCCATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGGGCCTTCTCAGTATTACTCGAGTAATACTGAGAAGGCCCATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGGGCCTTCTCAGTATTACTCGAGTAATACTGAGAAGGCCCATAG-3'

Other clones with same target sequence:

(none)