Construct: shRNA TRCN0000070843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010458.1-765s1c1
DNA Barcode:
ACCTACCAGTACCACTAGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hoxb3 (15410)
Gene Description:
homeobox B3
Transcript:
RefSeq NM_010458.1 (NON-CURRENT)
Match location:
Position 765 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTACCAGTACCACTAGCAA
Hairpin Sequence:
5'-CCGG-ACCTACCAGTACCACTAGCAA-CTCGAG-TTGCTAGTGGTACTGGTAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTACCAGTACCACTAGCAACTCGAGTTGCTAGTGGTACTGGTAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTACCAGTACCACTAGCAACTCGAGTTGCTAGTGGTACTGGTAGGT-3'

Other clones with same target sequence:

(none)