Construct: shRNA TRCN0000070873
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011912.1-844s1c1
- DNA Barcode:
- CCCTACAGCAGACTAGAACAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Vax2 (24113)
- Gene Description:
- ventral anterior homeobox 2
- Transcript:
- RefSeq NM_011912.1 (NON-CURRENT)
- Match location:
- Position 844 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000070873)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCCTACAGCAGACTAGAACAA
- Hairpin Sequence:
-
5'-CCGG-CCCTACAGCAGACTAGAACAA-CTCGAG-TTGTTCTAGTCTGCTGTAGGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCCTACAGCAGACTAGAACAACTCGAGTTGTTCTAGTCTGCTGTAGGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCCTACAGCAGACTAGAACAACTCGAGTTGTTCTAGTCTGCTGTAGGG-3'
Other clones with same target sequence:
(none)
