Construct: shRNA TRCN0000070878

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010461.1-1207s1c1
DNA Barcode:
CCCTTCGCAAATCCAGGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hoxb8 (15416)
Gene Description:
homeobox B8
Transcript:
RefSeq NM_010461.1 (NON-CURRENT)
Match location:
Position 1207 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070878)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCGCAAATCCAGGAGTT
Hairpin Sequence:
5'-CCGG-CCCTTCGCAAATCCAGGAGTT-CTCGAG-AACTCCTGGATTTGCGAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCGCAAATCCAGGAGTTCTCGAGAACTCCTGGATTTGCGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCGCAAATCCAGGAGTTCTCGAGAACTCCTGGATTTGCGAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000315975