Construct: shRNA TRCN0000070980

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010716.1-731s1c1
DNA Barcode:
CCAGTGAAAGGTGCTTCGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lig3 (16882)
Gene Description:
ligase III, DNA, ATP-dependent
Transcript:
RefSeq NM_010716.1 (NON-CURRENT)
Match location:
Position 731 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070980)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGAAAGGTGCTTCGTTT
Hairpin Sequence:
5'-CCGG-CCAGTGAAAGGTGCTTCGTTT-CTCGAG-AAACGAAGCACCTTTCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGAAAGGTGCTTCGTTTCTCGAGAAACGAAGCACCTTTCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGAAAGGTGCTTCGTTTCTCGAGAAACGAAGCACCTTTCACTGG-3'

Other clones with same target sequence:

  1. TRCN0000325437