Construct: shRNA TRCN0000070988

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009409.2-5346s1c1
DNA Barcode:
CCTTGTGTTGTCCTTTGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Top2b (21974)
Gene Description:
topoisomerase (DNA) II beta
Transcript:
RefSeq NM_009409.2 (CURRENT)
Match location:
Position 5346 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000070988)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGTGTTGTCCTTTGTCTT
Hairpin Sequence:
5'-CCGG-CCTTGTGTTGTCCTTTGTCTT-CTCGAG-AAGACAAAGGACAACACAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGTGTTGTCCTTTGTCTTCTCGAGAAGACAAAGGACAACACAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGTGTTGTCCTTTGTCTTCTCGAGAAGACAAAGGACAACACAAGG-3'

Other clones with same target sequence:

  1. TRCN0000349348