Construct: shRNA TRCN0000071013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011721.2-2314s1c1
DNA Barcode:
CGGCCAAATCTGTACTTAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wrn (22427)
Gene Description:
Werner syndrome RecQ like helicase
Transcript:
RefSeq NM_011721.2 (NON-CURRENT)
Match location:
Position 2314 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCCAAATCTGTACTTAGAA
Hairpin Sequence:
5'-CCGG-CGGCCAAATCTGTACTTAGAA-CTCGAG-TTCTAAGTACAGATTTGGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCCAAATCTGTACTTAGAACTCGAGTTCTAAGTACAGATTTGGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCCAAATCTGTACTTAGAACTCGAGTTCTAAGTACAGATTTGGCCG-3'

Other clones with same target sequence:

(none)