Construct: shRNA TRCN0000071014

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011721.2-4089s1c1
DNA Barcode:
CCCTCCCATCAACTCAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wrn (22427)
Gene Description:
Werner syndrome RecQ like helicase
Transcript:
RefSeq NM_011721.2 (NON-CURRENT)
Match location:
Position 4089 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071014)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCCCATCAACTCAGATAT
Hairpin Sequence:
5'-CCGG-CCCTCCCATCAACTCAGATAT-CTCGAG-ATATCTGAGTTGATGGGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCCCATCAACTCAGATATCTCGAGATATCTGAGTTGATGGGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCCCATCAACTCAGATATCTCGAGATATCTGAGTTGATGGGAGGG-3'

Other clones with same target sequence:

  1. TRCN0000335585