Construct: shRNA TRCN0000071019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178309.1-3156s1c1
DNA Barcode:
CGGCATTTCAATAGTAAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Brip1 (237911)
Gene Description:
BRCA1 interacting protein C-terminal helicase 1
Transcript:
RefSeq NM_178309.1 (NON-CURRENT)
Match location:
Position 3156 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCATTTCAATAGTAAAGTA
Hairpin Sequence:
5'-CCGG-CGGCATTTCAATAGTAAAGTA-CTCGAG-TACTTTACTATTGAAATGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCATTTCAATAGTAAAGTACTCGAGTACTTTACTATTGAAATGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCATTTCAATAGTAAAGTACTCGAGTACTTTACTATTGAAATGCCG-3'

Other clones with same target sequence:

(none)