Construct: shRNA TRCN0000071085

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025860.2-143s1c1
DNA Barcode:
CGAAACCTGAAGCTGCAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ddx18 (66942)
Gene Description:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
Transcript:
RefSeq NM_025860.2 (NON-CURRENT)
Match location:
Position 143 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071085)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAACCTGAAGCTGCAAGAA
Hairpin Sequence:
5'-CCGG-CGAAACCTGAAGCTGCAAGAA-CTCGAG-TTCTTGCAGCTTCAGGTTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAACCTGAAGCTGCAAGAACTCGAGTTCTTGCAGCTTCAGGTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAACCTGAAGCTGCAAGAACTCGAGTTCTTGCAGCTTCAGGTTTCG-3'

Other clones with same target sequence:

(none)