Construct: shRNA TRCN0000071115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007842.1-165s1c1
DNA Barcode:
GCCTATGAAATTAGAGCAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dhx9 (13211)
Gene Description:
DEAH (Asp-Glu-Ala-His) box polypeptide 9
Transcript:
RefSeq NM_007842.1 (NON-CURRENT)
Match location:
Position 165 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTATGAAATTAGAGCAGTA
Hairpin Sequence:
5'-CCGG-GCCTATGAAATTAGAGCAGTA-CTCGAG-TACTGCTCTAATTTCATAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTATGAAATTAGAGCAGTACTCGAGTACTGCTCTAATTTCATAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTATGAAATTAGAGCAGTACTCGAGTACTGCTCTAATTTCATAGGC-3'

Other clones with same target sequence:

(none)