Construct: shRNA TRCN0000071186

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011015.1-445s1c1
DNA Barcode:
GCACAGGAGATATTCTGGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Orc1 (18392)
Gene Description:
origin recognition complex, subunit 1
Transcript:
RefSeq NM_011015.1 (NON-CURRENT)
Match location:
Position 445 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071186)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGGAGATATTCTGGTAT
Hairpin Sequence:
5'-CCGG-GCACAGGAGATATTCTGGTAT-CTCGAG-ATACCAGAATATCTCCTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGGAGATATTCTGGTATCTCGAGATACCAGAATATCTCCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGGAGATATTCTGGTATCTCGAGATACCAGAATATCTCCTGTGC-3'

Other clones with same target sequence:

(none)