Construct: shRNA TRCN0000071226

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028228.1-999s1c1
DNA Barcode:
GTAGAAATAGACGCCACACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pinx1 (72400)
Gene Description:
PIN2/TERF1 interacting, telomerase inhibitor 1
Transcript:
RefSeq NM_028228.1 (NON-CURRENT)
Match location:
Position 999 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071226)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGAAATAGACGCCACACTA
Hairpin Sequence:
5'-CCGG-GTAGAAATAGACGCCACACTA-CTCGAG-TAGTGTGGCGTCTATTTCTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGAAATAGACGCCACACTACTCGAGTAGTGTGGCGTCTATTTCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGAAATAGACGCCACACTACTCGAGTAGTGTGGCGTCTATTTCTAC-3'

Other clones with same target sequence:

(none)