Construct: shRNA TRCN0000071238

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017462.1-3711s1c1
DNA Barcode:
GCCTATAAGCTGGGTCTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Polg (18975)
Gene Description:
polymerase (DNA directed), gamma
Transcript:
RefSeq NM_017462.1 (NON-CURRENT)
Match location:
Position 3711 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071238)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTATAAGCTGGGTCTGAAT
Hairpin Sequence:
5'-CCGG-GCCTATAAGCTGGGTCTGAAT-CTCGAG-ATTCAGACCCAGCTTATAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTATAAGCTGGGTCTGAATCTCGAGATTCAGACCCAGCTTATAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTATAAGCTGGGTCTGAATCTCGAGATTCAGACCCAGCTTATAGGC-3'

Other clones with same target sequence:

(none)