Construct: shRNA TRCN0000071322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_148948.1-2744s1c1
DNA Barcode:
CGAGCTGAAGAAGTCTGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dicer1 (192119)
Gene Description:
dicer 1, ribonuclease type III
Transcript:
RefSeq NM_148948.1 (NON-CURRENT)
Match location:
Position 2744 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCTGAAGAAGTCTGGTTT
Hairpin Sequence:
5'-CCGG-CGAGCTGAAGAAGTCTGGTTT-CTCGAG-AAACCAGACTTCTTCAGCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCTGAAGAAGTCTGGTTTCTCGAGAAACCAGACTTCTTCAGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCTGAAGAAGTCTGGTTTCTCGAGAAACCAGACTTCTTCAGCTCG-3'

Other clones with same target sequence:

  1. TRCN0000331536