Construct: shRNA TRCN0000071339

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175238.3-2590s1c1
DNA Barcode:
CCCTCTATGATCCGAGAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rif1 (51869)
Gene Description:
replication timing regulatory factor 1
Transcript:
RefSeq NM_175238.3 (NON-CURRENT)
Match location:
Position 2590 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071339)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCTATGATCCGAGAAATA
Hairpin Sequence:
5'-CCGG-CCCTCTATGATCCGAGAAATA-CTCGAG-TATTTCTCGGATCATAGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCTATGATCCGAGAAATACTCGAGTATTTCTCGGATCATAGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCTATGATCCGAGAAATACTCGAGTATTTCTCGGATCATAGAGGG-3'

Other clones with same target sequence:

(none)