Construct: shRNA TRCN0000071376

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133872.1-1863s1c1
DNA Barcode:
GCTGAAGGCTTGGACATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kdm1a (99982)
Gene Description:
lysine (K)-specific demethylase 1A
Transcript:
RefSeq NM_133872.1 (NON-CURRENT)
Match location:
Position 1863 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071376)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAGGCTTGGACATTAAA
Hairpin Sequence:
5'-CCGG-GCTGAAGGCTTGGACATTAAA-CTCGAG-TTTAATGTCCAAGCCTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAGGCTTGGACATTAAACTCGAGTTTAATGTCCAAGCCTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAGGCTTGGACATTAAACTCGAGTTTAATGTCCAAGCCTTCAGC-3'

Other clones with same target sequence:

(none)