Construct: shRNA TRCN0000071379

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008114.2-216s1c1
DNA Barcode:
CTGCTGTAATTCCTGTGGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gfi1b (14582)
Gene Description:
growth factor independent 1B
Transcript:
RefSeq NM_008114.2 (NON-CURRENT)
Match location:
Position 216 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071379)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCTGTAATTCCTGTGGCAA
Hairpin Sequence:
5'-CCGG-CTGCTGTAATTCCTGTGGCAA-CTCGAG-TTGCCACAGGAATTACAGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCTGTAATTCCTGTGGCAACTCGAGTTGCCACAGGAATTACAGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCTGTAATTCCTGTGGCAACTCGAGTTGCCACAGGAATTACAGCAG-3'

Other clones with same target sequence:

(none)