Construct: shRNA TRCN0000071381

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008114.2-260s1c1
DNA Barcode:
CCTCTTCTCAGCACACCGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gfi1b (14582)
Gene Description:
growth factor independent 1B
Transcript:
RefSeq NM_008114.2 (NON-CURRENT)
Match location:
Position 260 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071381)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTCTCAGCACACCGCTT
Hairpin Sequence:
5'-CCGG-CCTCTTCTCAGCACACCGCTT-CTCGAG-AAGCGGTGTGCTGAGAAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTCTCAGCACACCGCTTCTCGAGAAGCGGTGTGCTGAGAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTCTCAGCACACCGCTTCTCGAGAAGCGGTGTGCTGAGAAGAGG-3'

Other clones with same target sequence:

(none)