Construct: shRNA TRCN0000071397

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033566.1-5663s1c1
DNA Barcode:
CATTGGTTTCACAAGTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arid1a (93760)
Gene Description:
AT rich interactive domain 1A (SWI-like)
Transcript:
RefSeq NM_033566.1 (NON-CURRENT)
Match location:
Position 5663 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071397)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTGGTTTCACAAGTCATTT
Hairpin Sequence:
5'-CCGG-CATTGGTTTCACAAGTCATTT-CTCGAG-AAATGACTTGTGAAACCAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTGGTTTCACAAGTCATTTCTCGAGAAATGACTTGTGAAACCAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTGGTTTCACAAGTCATTTCTCGAGAAATGACTTGTGAAACCAATG-3'

Other clones with same target sequence:

(none)