Construct: shRNA TRCN0000071422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146081.1-761s1c1
DNA Barcode:
GTAACATAAGTCACGACACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp4r1 (70351)
Gene Description:
protein phosphatase 4, regulatory subunit 1
Transcript:
RefSeq NM_146081.1 (NON-CURRENT)
Match location:
Position 761 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAACATAAGTCACGACACTT
Hairpin Sequence:
5'-CCGG-GTAACATAAGTCACGACACTT-CTCGAG-AAGTGTCGTGACTTATGTTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAACATAAGTCACGACACTTCTCGAGAAGTGTCGTGACTTATGTTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAACATAAGTCACGACACTTCTCGAGAAGTGTCGTGACTTATGTTAC-3'

Other clones with same target sequence:

(none)