Construct: shRNA TRCN0000071534

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024427.2-806s1c1
DNA Barcode:
GCAGAGAGATCAGTAACCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tpm1 (22003)
Gene Description:
tropomyosin 1, alpha
Transcript:
RefSeq NM_024427.2 (NON-CURRENT)
Match location:
Position 806 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071534)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGAGATCAGTAACCAAA
Hairpin Sequence:
5'-CCGG-GCAGAGAGATCAGTAACCAAA-CTCGAG-TTTGGTTACTGATCTCTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGAGATCAGTAACCAAACTCGAGTTTGGTTACTGATCTCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGAGATCAGTAACCAAACTCGAGTTTGGTTACTGATCTCTCTGC-3'

Other clones with same target sequence:

(none)