Construct: shRNA TRCN0000071587

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020587.1-785s1c1
DNA Barcode:
GTGTCAAAGGAGCGCGAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srsf4 (57317)
Gene Description:
serine/arginine-rich splicing factor 4
Transcript:
RefSeq NM_020587.1 (NON-CURRENT)
Match location:
Position 785 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071587)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTCAAAGGAGCGCGAACAT
Hairpin Sequence:
5'-CCGG-GTGTCAAAGGAGCGCGAACAT-CTCGAG-ATGTTCGCGCTCCTTTGACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTCAAAGGAGCGCGAACATCTCGAGATGTTCGCGCTCCTTTGACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTCAAAGGAGCGCGAACATCTCGAGATGTTCGCGCTCCTTTGACAC-3'

Other clones with same target sequence:

  1. TRCN0000327131