Construct: shRNA TRCN0000071623

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019641.2-565s1c1
DNA Barcode:
CCTAAATATCCAAAGACTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stmn1 (16765)
Gene Description:
stathmin 1
Transcript:
RefSeq NM_019641.2 (NON-CURRENT)
Match location:
Position 565 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071623)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAATATCCAAAGACTGTA
Hairpin Sequence:
5'-CCGG-CCTAAATATCCAAAGACTGTA-CTCGAG-TACAGTCTTTGGATATTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAATATCCAAAGACTGTACTCGAGTACAGTCTTTGGATATTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAATATCCAAAGACTGTACTCGAGTACAGTCTTTGGATATTTAGG-3'

Other clones with same target sequence:

(none)