Construct: shRNA TRCN0000071708

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175172.2-3168s1c1
DNA Barcode:
CCACGGTGAATAAATAGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shtn1 (71653)
Gene Description:
shootin 1
Transcript:
RefSeq NM_175172.2 (NON-CURRENT)
Match location:
Position 3168 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071708)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGGTGAATAAATAGAAAT
Hairpin Sequence:
5'-CCGG-CCACGGTGAATAAATAGAAAT-CTCGAG-ATTTCTATTTATTCACCGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGGTGAATAAATAGAAATCTCGAGATTTCTATTTATTCACCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGGTGAATAAATAGAAATCTCGAGATTTCTATTTATTCACCGTGG-3'

Other clones with same target sequence:

(none)