Construct: shRNA TRCN0000071719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022982.1-1308s1c1
DNA Barcode:
GCACATCAATGACTCTCCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rtn4r (65079)
Gene Description:
reticulon 4 receptor
Transcript:
RefSeq NM_022982.1 (NON-CURRENT)
Match location:
Position 1308 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATCAATGACTCTCCATT
Hairpin Sequence:
5'-CCGG-GCACATCAATGACTCTCCATT-CTCGAG-AATGGAGAGTCATTGATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATCAATGACTCTCCATTCTCGAGAATGGAGAGTCATTGATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATCAATGACTCTCCATTCTCGAGAATGGAGAGTCATTGATGTGC-3'

Other clones with same target sequence:

(none)