Construct: shRNA TRCN0000071749

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015814.2-1028s1c1
DNA Barcode:
CCGGATGAGTACGAAGATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dkk3 (50781)
Gene Description:
dickkopf WNT signaling pathway inhibitor 3
Transcript:
RefSeq NM_015814.2 (CURRENT)
Match location:
Position 1028 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071749)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGATGAGTACGAAGATGTT
Hairpin Sequence:
5'-CCGG-CCGGATGAGTACGAAGATGTT-CTCGAG-AACATCTTCGTACTCATCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGATGAGTACGAAGATGTTCTCGAGAACATCTTCGTACTCATCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGATGAGTACGAAGATGTTCTCGAGAACATCTTCGTACTCATCCGG-3'

Other clones with same target sequence:

  1. TRCN0000352025