Construct: shRNA TRCN0000071780

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009528.2-297s1c1
DNA Barcode:
ACCCGATGCCATCATTGTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wnt7b (22422)
Gene Description:
wingless-type MMTV integration site family, member 7B
Transcript:
RefSeq NM_009528.2 (NON-CURRENT)
Match location:
Position 297 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071780)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCGATGCCATCATTGTGAT
Hairpin Sequence:
5'-CCGG-ACCCGATGCCATCATTGTGAT-CTCGAG-ATCACAATGATGGCATCGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCGATGCCATCATTGTGATCTCGAGATCACAATGATGGCATCGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCGATGCCATCATTGTGATCTCGAGATCACAATGATGGCATCGGGT-3'

Other clones with same target sequence:

(none)