Construct: shRNA TRCN0000071789

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009527.2-360s1c1
DNA Barcode:
TGCGAGCATCATCTGTAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wnt7a (22421)
Gene Description:
wingless-type MMTV integration site family, member 7A
Transcript:
RefSeq NM_009527.2 (NON-CURRENT)
Match location:
Position 360 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071789)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGAGCATCATCTGTAACAA
Hairpin Sequence:
5'-CCGG-TGCGAGCATCATCTGTAACAA-CTCGAG-TTGTTACAGATGATGCTCGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGAGCATCATCTGTAACAACTCGAGTTGTTACAGATGATGCTCGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGAGCATCATCTGTAACAACTCGAGTTGTTACAGATGATGCTCGCA-3'

Other clones with same target sequence:

(none)