Construct: shRNA TRCN0000071905

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009472.1-265s1c1
DNA Barcode:
GCCGCTCAAGATGATGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Unc5c (22253)
Gene Description:
unc-5 netrin receptor C
Transcript:
RefSeq NM_009472.1 (NON-CURRENT)
Match location:
Position 265 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071905)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCTCAAGATGATGAATTT
Hairpin Sequence:
5'-CCGG-GCCGCTCAAGATGATGAATTT-CTCGAG-AAATTCATCATCTTGAGCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCTCAAGATGATGAATTTCTCGAGAAATTCATCATCTTGAGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCTCAAGATGATGAATTTCTCGAGAAATTCATCATCTTGAGCGGC-3'

Other clones with same target sequence:

(none)