Construct: shRNA TRCN0000071935

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146083.1-284s1c1
DNA Barcode:
GTTCGAGGATTGGATGGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srsf7 (225027)
Gene Description:
serine/arginine-rich splicing factor 7
Transcript:
RefSeq NM_146083.1 (NON-CURRENT)
Match location:
Position 284 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071935)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCGAGGATTGGATGGGAAA
Hairpin Sequence:
5'-CCGG-GTTCGAGGATTGGATGGGAAA-CTCGAG-TTTCCCATCCAATCCTCGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCGAGGATTGGATGGGAAACTCGAGTTTCCCATCCAATCCTCGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCGAGGATTGGATGGGAAACTCGAGTTTCCCATCCAATCCTCGAAC-3'

Other clones with same target sequence:

  1. TRCN0000324747