Construct: shRNA TRCN0000071940

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011595.1-634s1c1
DNA Barcode:
GCGCGTGTATGAAGGCAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Timp3 (21859)
Gene Description:
tissue inhibitor of metalloproteinase 3
Transcript:
RefSeq NM_011595.1 (NON-CURRENT)
Match location:
Position 634 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071940)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCGTGTATGAAGGCAAGAT
Hairpin Sequence:
5'-CCGG-GCGCGTGTATGAAGGCAAGAT-CTCGAG-ATCTTGCCTTCATACACGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCGTGTATGAAGGCAAGATCTCGAGATCTTGCCTTCATACACGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCGTGTATGAAGGCAAGATCTCGAGATCTTGCCTTCATACACGCGC-3'

Other clones with same target sequence:

  1. TRCN0000324755