Construct: shRNA TRCN0000071951

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011594.2-462s1c1
DNA Barcode:
CCTGACAAAGACATCGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Timp2 (21858)
Gene Description:
tissue inhibitor of metalloproteinase 2
Transcript:
RefSeq NM_011594.2 (NON-CURRENT)
Match location:
Position 462 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071951)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGACAAAGACATCGAGTTT
Hairpin Sequence:
5'-CCGG-CCTGACAAAGACATCGAGTTT-CTCGAG-AAACTCGATGTCTTTGTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGACAAAGACATCGAGTTTCTCGAGAAACTCGATGTCTTTGTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGACAAAGACATCGAGTTTCTCGAGAAACTCGATGTCTTTGTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000324746