Construct: shRNA TRCN0000071956

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144850.1-1245s1c1
DNA Barcode:
GCCACCATCATCCTTCGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rapgef3 (223864)
Gene Description:
Rap guanine nucleotide exchange factor (GEF) 3
Transcript:
RefSeq NM_144850.1 (NON-CURRENT)
Match location:
Position 1245 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000071956)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCATCATCCTTCGAGAA
Hairpin Sequence:
5'-CCGG-GCCACCATCATCCTTCGAGAA-CTCGAG-TTCTCGAAGGATGATGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCATCATCCTTCGAGAACTCGAGTTCTCGAAGGATGATGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCATCATCCTTCGAGAACTCGAGTTCTCGAAGGATGATGGTGGC-3'

Other clones with same target sequence:

(none)