Construct: shRNA TRCN0000072035

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009115.2-262s1c1
DNA Barcode:
GAGCAGGAAGTGGTGGACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
S100b (20203)
Gene Description:
S100 protein, beta polypeptide, neural
Transcript:
RefSeq NM_009115.2 (NON-CURRENT)
Match location:
Position 262 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072035)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAGGAAGTGGTGGACAAA
Hairpin Sequence:
5'-CCGG-GAGCAGGAAGTGGTGGACAAA-CTCGAG-TTTGTCCACCACTTCCTGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAGGAAGTGGTGGACAAACTCGAGTTTGTCCACCACTTCCTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAGGAAGTGGTGGACAAACTCGAGTTTGTCCACCACTTCCTGCTC-3'

Other clones with same target sequence:

(none)