Construct: shRNA TRCN0000072049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011294.2-250s1c1
DNA Barcode:
CCAGATTGGAAAGATGAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sub1 (20024)
Gene Description:
SUB1 homolog (S. cerevisiae)
Transcript:
RefSeq NM_011294.2 (NON-CURRENT)
Match location:
Position 250 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATTGGAAAGATGAGATA
Hairpin Sequence:
5'-CCGG-CCAGATTGGAAAGATGAGATA-CTCGAG-TATCTCATCTTTCCAATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATTGGAAAGATGAGATACTCGAGTATCTCATCTTTCCAATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATTGGAAAGATGAGATACTCGAGTATCTCATCTTTCCAATCTGG-3'

Other clones with same target sequence:

  1. TRCN0000302263