Construct: shRNA TRCN0000072108

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199007.1-4685s1c1
DNA Barcode:
GCTCGTATCTATCTACTGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sgo2a (68549)
Gene Description:
shugoshin 2A
Transcript:
RefSeq NM_199007.1 (NON-CURRENT)
Match location:
Position 4685 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072108)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGTATCTATCTACTGATT
Hairpin Sequence:
5'-CCGG-GCTCGTATCTATCTACTGATT-CTCGAG-AATCAGTAGATAGATACGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGTATCTATCTACTGATTCTCGAGAATCAGTAGATAGATACGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGTATCTATCTACTGATTCTCGAGAATCAGTAGATAGATACGAGC-3'

Other clones with same target sequence:

(none)