Construct: shRNA TRCN0000072119

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001014996.1-863s1c1
DNA Barcode:
CCACTCAAGATTCCGTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cenpj (219103)
Gene Description:
centromere protein J
Transcript:
RefSeq NM_001014996.1 (NON-CURRENT)
Match location:
Position 863 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072119)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCAAGATTCCGTATTAA
Hairpin Sequence:
5'-CCGG-CCACTCAAGATTCCGTATTAA-CTCGAG-TTAATACGGAATCTTGAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCAAGATTCCGTATTAACTCGAGTTAATACGGAATCTTGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCAAGATTCCGTATTAACTCGAGTTAATACGGAATCTTGAGTGG-3'

Other clones with same target sequence:

(none)