Construct: shRNA TRCN0000072124

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008712.1-1470s1c1
DNA Barcode:
GCCATCACTATATTCCCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nos1 (18125)
Gene Description:
nitric oxide synthase 1, neuronal
Transcript:
RefSeq NM_008712.1 (NON-CURRENT)
Match location:
Position 1470 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072124)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCACTATATTCCCTCAA
Hairpin Sequence:
5'-CCGG-GCCATCACTATATTCCCTCAA-CTCGAG-TTGAGGGAATATAGTGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCACTATATTCCCTCAACTCGAGTTGAGGGAATATAGTGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCACTATATTCCCTCAACTCGAGTTGAGGGAATATAGTGATGGC-3'

Other clones with same target sequence:

(none)